abstract：:Wnt signaling is intrinsic to mouse embryonic stem cell self-renewal. Therefore, it is surprising that reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is not strongly enhanced by Wnt signaling. Here, we demonstrate that active Wnt signaling inhibits the early stage of reprogramming to iPSCs, w...

journal_title：Cell reports

authors： Ho R,Papp B,Hoffman JA,Merrill BJ,Plath K

更新日期：2013-06-27 00:00:00

abstract：:The mechanisms by which p53 suppresses tumor growth remain ill defined. In this issue of Cell Reports, Timofeev et al. (2013) and Valente et al. (2013) reveal context-dependent contributions of p53-dependent apoptosis to its tumor-suppressive function. ...

journal_title：Cell reports

authors： Mellert H,Espinosa JM

更新日期：2013-05-30 00:00:00

abstract：:Neutrophil polarity relies on local, mutual inhibition to segregate incompatible signaling circuits to the leading and trailing edges. Mutual inhibition alone should lead to cells having strong fronts and weak backs or vice versa. However, analysis of cell-to-cell variation in human neutrophils revealed that back pola...

journal_title：Cell reports

authors： Wang Y,Ku CJ,Zhang ER,Artyukhin AB,Weiner OD,Wu LF,Altschuler SJ

更新日期：2013-05-30 00:00:00

abstract：:GPR116 is an orphan seven-pass transmembrane receptor whose function has been unclear. Global disruption of the Gpr116 gene in mice revealed an unexpected, critical role for this receptor in lung surfactant homeostasis, resulting in progressive accumulation of surfactant lipids and proteins in the alveolar space, labo...

journal_title：Cell reports

authors： Yang MY,Hilton MB,Seaman S,Haines DC,Nagashima K,Burks CM,Tessarollo L,Ivanova PT,Brown HA,Umstead TM,Floros J,Chroneos ZC,St Croix B

更新日期：2013-05-30 00:00:00

abstract：:The transcriptional coactivators PGC-1α and PGC-1β are widely thought to be required for mitochondrial biogenesis and fiber typing in skeletal muscle. Here, we show that mice lacking both PGC-1s in myocytes do indeed have profoundly deficient mitochondrial respiration but, surprisingly, have preserved mitochondrial co...

journal_title：Cell reports

authors： Rowe GC,Patten IS,Zsengeller ZK,El-Khoury R,Okutsu M,Bampoh S,Koulisis N,Farrell C,Hirshman MF,Yan Z,Goodyear LJ,Rustin P,Arany Z

更新日期：2013-05-30 00:00:00

abstract：:The vitamin A metabolite retinoic acid (RA) provides patterning information during vertebrate embryogenesis, but the mechanism through which RA influences limb development is unclear. During patterning of the limb proximodistal axis (upper limb to digits), avian studies suggest that a proximal RA signal generated in t...

journal_title：Cell reports

authors： Cunningham TJ,Zhao X,Sandell LL,Evans SM,Trainor PA,Duester G

更新日期：2013-05-30 00:00:00

abstract：:Transcription-factor-induced reprogramming of somatic cells to pluripotency is a very inefficient process, probably due to the existence of important epigenetic barriers that are imposed during differentiation and that contribute to preserving cell identity. In an effort to decipher the molecular nature of these barri...

journal_title：Cell reports

authors： Barrero MJ,Sese B,Kuebler B,Bilic J,Boue S,Martí M,Izpisua Belmonte JC

更新日期：2013-04-25 00:00:00

abstract：:DNA methylation provides a mechanism by which environmental factors can control insulin sensitivity in obesity. Here, we assessed DNA methylation in skeletal muscle from obese people before and after Roux-en-Y gastric bypass (RYGB). Obesity was associated with altered expression of a subset of genes enriched in metabo...

journal_title：Cell reports

authors： Barres R,Kirchner H,Rasmussen M,Yan J,Kantor FR,Krook A,Näslund E,Zierath JR

更新日期：2013-04-25 00:00:00

abstract：:Synapse formation is a complex process that involves the recruitment and assembly of a myriad of pre- and postsynaptic proteins. Despite being present at every synapse in the vertebrate CNS, little is known about the transport, recruitment, and stabilization of synapsin at nascent synapses during development. We exami...

journal_title：Cell reports

authors： Easley-Neal C,Fierro J Jr,Buchanan J,Washbourne P

更新日期：2013-04-25 00:00:00

abstract：:During an infection the antigen-nonspecific memory CD8 T cell compartment is not simply an inert pool of cells, but becomes activated and cytotoxic. It is unknown how these cells contribute to the clearance of an infection. We measured the strength of T cell receptor (TCR) signals that bystander-activated, cytotoxic C...

journal_title：Cell reports

authors： Chu T,Tyznik AJ,Roepke S,Berkley AM,Woodward-Davis A,Pattacini L,Bevan MJ,Zehn D,Prlic M

更新日期：2013-03-28 00:00:00

abstract：:A new influenza-like virus genome (H17N10) was recently discovered in bats and offers a new perspective about the origin and evolution of influenza viruses. The viral envelope glycoprotein hemagglutinin (HA) is responsible for influenza virus receptor binding, fusion, and entry into the cell; therefore, the structure ...

journal_title：Cell reports

authors： Sun X,Shi Y,Lu X,He J,Gao F,Yan J,Qi J,Gao GF

更新日期：2013-03-28 00:00:00

abstract：:Mutations in WNK kinases cause the human hypertensive disease pseudohypoaldosteronism type II (PHAII), but the regulatory mechanisms of the WNK kinases are not well understood. Mutations in kelch-like 3 (KLHL3) and Cullin3 were also recently identified as causing PHAII. Therefore, new insights into the mechanisms of h...

journal_title：Cell reports

authors： Wakabayashi M,Mori T,Isobe K,Sohara E,Susa K,Araki Y,Chiga M,Kikuchi E,Nomura N,Mori Y,Matsuo H,Murata T,Nomura S,Asano T,Kawaguchi H,Nonoyama S,Rai T,Sasaki S,Uchida S

更新日期：2013-03-28 00:00:00

abstract：:Plasmodium parasites infect the liver and replicate inside hepatocytes before they invade erythrocytes and trigger clinical malaria. Analysis of host signaling pathways affected by liver-stage infection could provide critical insights into host-pathogen interactions and reveal targets for intervention. Using protein l...

journal_title：Cell reports

authors： Kaushansky A,Ye AS,Austin LS,Mikolajczak SA,Vaughan AM,Camargo N,Metzger PG,Douglass AN,MacBeath G,Kappe SH

更新日期：2013-03-28 00:00:00

abstract：:Amphotericin, miconazole, and ciclopirox are antifungal agents from three different drug classes that can effectively kill planktonic yeast, yet their complete fungicidal mechanisms are not fully understood. Here, we employ a systems biology approach to identify a common oxidative-damage cellular death pathway trigger...

journal_title：Cell reports

authors： Belenky P,Camacho D,Collins JJ

更新日期：2013-02-21 00:00:00

abstract：:Activation of the transcription factor NF-κB is essential for innate immune function and requires strict regulation. The micronutrient zinc modulates proper host defense, and zinc deficiency is associated with elevated inflammation and worse outcomes in response to bacterial infection and sepsis. Previous studies sugg...

journal_title：Cell reports

authors： Liu MJ,Bao S,Gálvez-Peralta M,Pyle CJ,Rudawsky AC,Pavlovicz RE,Killilea DW,Li C,Nebert DW,Wewers MD,Knoell DL

更新日期：2013-02-21 00:00:00

abstract：:Chromosomes with multiple DNA replication origins are a hallmark of Eukaryotes and some Archaea. All eukaryal nuclear replication origins are defined by the origin recognition complex (ORC) that recruits the replicative helicase MCM(2-7) via Cdc6 and Cdt1. We find that the three origins in the single chromosome of the...

journal_title：Cell reports

authors： Samson RY,Xu Y,Gadelha C,Stone TA,Faqiri JN,Li D,Qin N,Pu F,Liang YX,She Q,Bell SD

更新日期：2013-02-21 00:00:00

abstract：:Gambogic acid (GA) is a natural compound derived from Chinese herbs that has been approved by the Chinese Food and Drug Administration for clinical trials in cancer patients; however, its molecular targets have not been thoroughly studied. Here, we report that GA inhibits tumor proteasome activity, with potency compar...

journal_title：Cell reports

authors： Li X,Liu S,Huang H,Liu N,Zhao C,Liao S,Yang C,Liu Y,Zhao C,Li S,Lu X,Liu C,Guan L,Zhao K,Shi X,Song W,Zhou P,Dong X,Guo H,Wen G,Zhang C,Jiang L,Ma N,Li B,Wang S,Tan H,Wang X,Dou QP,Liu J

更新日期：2013-01-31 00:00:00

abstract：:Chromodomain Helicase DNA binding protein 5 (CHD5) is a tumor suppressor mapping to 1p36, a genomic region that is frequently deleted in human cancer. Although CHD5 belongs to the CHD family of chromatin-remodeling proteins, whether its tumor-suppressive role involves an interaction with chromatin is unknown. Here we ...

journal_title：Cell reports

authors： Paul S,Kuo A,Schalch T,Vogel H,Joshua-Tor L,McCombie WR,Gozani O,Hammell M,Mills AA

更新日期：2013-01-31 00:00:00

abstract：:Insulin stimulates glucose uptake through the membrane translocation of GLUT4 and GLUT1. Peroxisome proliferator-activated receptor γ (PPARγ) enhances insulin sensitivity. Here, we demonstrate that insulin stimulates GLUT4 and GLUT1 translocation, and glucose uptake, by activating the signaling pathway involving nicot...

journal_title：Cell reports

authors： Song EK,Lee YR,Kim YR,Yeom JH,Yoo CH,Kim HK,Park HM,Kang HS,Kim JS,Kim UH,Han MK

更新日期：2012-12-27 00:00:00

abstract：:The efficient generation of hematopoietic stem cells from human pluripotent stem cells is dependent on the appropriate specification of the definitive hematopoietic program during differentiation. In this study, we used T lymphocyte potential to track the onset of definitive hematopoiesis from human embryonic and indu...

journal_title：Cell reports

authors： Kennedy M,Awong G,Sturgeon CM,Ditadi A,LaMotte-Mohs R,Zúñiga-Pflücker JC,Keller G

更新日期：2012-12-27 00:00:00

abstract：:After implantation, pluripotent epiblasts are converted to embryonic ectoderm through cell-cell interactions that significantly change the transcriptional and epigenetic networks. An entrée to understanding this vital developmental transition is the t(w5) mutation of the mouse t complex. This mutation produces highly ...

journal_title：Cell reports

authors： Sugimoto M,Kondo M,Hirose M,Suzuki M,Mekada K,Abe T,Kiyonari H,Ogura A,Takagi N,Artzt K,Abe K

更新日期：2012-11-29 00:00:00

abstract：:Chromatin interactions play important roles in transcription regulation. To better understand the underlying evolutionary and functional constraints of these interactions, we implemented a systems approach to examine RNA polymerase-II-associated chromatin interactions in human cells. We found that 40% of the total gen...

journal_title：Cell reports

authors： Sandhu KS,Li G,Poh HM,Quek YL,Sia YY,Peh SQ,Mulawadi FH,Lim J,Sikic M,Menghi F,Thalamuthu A,Sung WK,Ruan X,Fullwood MJ,Liu E,Csermely P,Ruan Y

更新日期：2012-11-29 00:00:00

abstract：:Senescence is a cellular response preventing tumorigenesis. The Ras oncogene is frequently activated or mutated in human cancers, but Ras activation is insufficient to transform primary cells. In a search for cooperating oncogenes, we identify the lysine demethylase JMJD2A/KDM4A. We show that JMJD2A functions as a neg...

journal_title：Cell reports

authors： Mallette FA,Richard S

更新日期：2012-11-29 00:00:00

abstract：:Sustained canonical Wnt signaling requires the inhibition of glycogen synthase kinase 3 (GSK3) activity by sequestration of GSK3 inside multivesicular endosomes (MVEs). Here, we show that Wnt signaling is increased by the lysosomal inhibitor chloroquine, which causes accumulation of MVEs. A similar MVE expansion and i...

journal_title：Cell reports

authors： Dobrowolski R,Vick P,Ploper D,Gumper I,Snitkin H,Sabatini DD,De Robertis EM

更新日期：2012-11-29 00:00:00

abstract：:Mutation hotspots and showers occur across phylogeny and profoundly influence genome evolution, yet the mechanisms that produce hotspots remain obscure. We report that DNA double-strand breaks (DSBs) provoke mutation hotspots via stress-induced mutation in Escherichia coli. With tet reporters placed 2 kb to 2 Mb (half...

journal_title：Cell reports

authors： Shee C,Gibson JL,Rosenberg SM

更新日期：2012-10-25 00:00:00

abstract：:Clericuzio-type poikiloderma with neutropenia (PN) is a rare genodermatosis associated with mutations in the C16orf57 gene, which codes for the uncharacterized protein hMpn1. We show here that, in both fission yeasts and humans, Mpn1 processes the spliceosomal U6 small nuclear RNA (snRNA) posttranscriptionally. In Mpn...

journal_title：Cell reports

authors： Shchepachev V,Wischnewski H,Missiaglia E,Soneson C,Azzalin CM

更新日期：2012-10-25 00:00:00

abstract：:A two-step, automated, high-throughput RNAi silencing screen was used to identify host cell factors required during vaccinia virus infection. Validation and analysis of clustered hits revealed previously unknown processes during virus entry, including a mechanism for genome uncoating. Viral core proteins were found to...

journal_title：Cell reports

authors： Mercer J,Snijder B,Sacher R,Burkard C,Bleck CK,Stahlberg H,Pelkmans L,Helenius A

更新日期：2012-10-25 00:00:00

abstract：:Transgenic zebrafish embryos expressing tissue-specific green fluorescent protein (GFP) can provide an unlimited supply of primary embryonic cells. Agents that promote the differentiation of these cells may be beneficial for therapeutics. We report a high-throughput approach for screening small molecules that regulate...

journal_title：Cell reports

authors： Huang H,Lindgren A,Wu X,Liu NA,Lin S

更新日期：2012-09-27 00:00:00

abstract：:The notion that decapping leads irreversibly to messenger RNA (mRNA) decay was contradicted by the identification of capped transcripts missing portions of their 5' ends and a cytoplasmic complex that can restore the cap on uncapped mRNAs. In this study, we used accumulation of uncapped transcripts in cells inhibited ...

journal_title：Cell reports

authors： Mukherjee C,Patil DP,Kennedy BA,Bakthavachalu B,Bundschuh R,Schoenberg DR

更新日期：2012-09-27 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a devastating disease that progresses from detachment of motor nerve terminals to complete muscle paralysis and lethal respiratory failure within 5 years of diagnosis. Genetic studies have linked mutations in several genes to ALS, and mice bearing mutations in SOD1 recapitulate h...

journal_title：Cell reports

authors： Pérez-García MJ,Burden SJ

更新日期：2012-09-27 00:00:00

abstract：:Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data. Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cycle and lineage cha...

journal_title：Cell reports

authors： Berger C,Harzer H,Burkard TR,Steinmann J,van der Horst S,Laurenson AS,Novatchkova M,Reichert H,Knoblich JA

更新日期：2012-08-30 00:00:00

abstract：:One puzzling observation in patients affected with Hutchinson-Gilford progeria syndrome (HGPS), who overall exhibit systemic and dramatic premature aging, is the absence of any conspicuous cognitive impairment. Recent studies based on induced pluripotent stem cells derived from HGPS patient cells have revealed a lack ...

journal_title：Cell reports

authors： Nissan X,Blondel S,Navarro C,Maury Y,Denis C,Girard M,Martinat C,De Sandre-Giovannoli A,Levy N,Peschanski M

更新日期：2012-07-26 00:00:00

abstract：:In neurons, transmembrane proteins are targeted to dendrites in vesicles that traffic solely within the somatodendritic compartment. How these vesicles are retained within the somatodendritic domain is unknown. Here, we use a novel pulse-chase system, which allows synchronous release of exogenous transmembrane protein...

journal_title：Cell reports

authors： Al-Bassam S,Xu M,Wandless TJ,Arnold DB

更新日期：2012-07-26 00:00:00

abstract：:Chromothripsis represents a novel phenomenon in the structural variation landscape of cancer genomes. Here, we analyze the genomes of ten patients with congenital disease who were preselected to carry complex chromosomal rearrangements with more than two breakpoints. The rearrangements displayed unanticipated complexi...

journal_title：Cell reports

authors： Kloosterman WP,Tavakoli-Yaraki M,van Roosmalen MJ,van Binsbergen E,Renkens I,Duran K,Ballarati L,Vergult S,Giardino D,Hansson K,Ruivenkamp CA,Jager M,van Haeringen A,Ippel EF,Haaf T,Passarge E,Hochstenbach R,Menten B,

更新日期：2012-06-28 00:00:00

abstract：:During cell division, the molecular motor Eg5 crosslinks overlapping antiparallel microtubules and pushes them apart to separate mitotic spindle poles. Dynein has been proposed as a direct antagonist of Eg5 at the spindle equator, pulling on antiparallel microtubules and favoring spindle collapse. Some of the experime...

journal_title：Cell reports

authors： Florian S,Mayer TU

更新日期：2012-05-31 00:00:00

abstract：:Oct4 is an essential regulator of pluripotency in vivo and in vitro in embryonic stem cells, as well as a key mediator of the reprogramming of somatic cells into induced pluripotent stem cells. It is not known whether activation and/or repression of specific genes by Oct4 is relevant to these functions. Here, we show ...

journal_title：Cell reports

authors： Hammachi F,Morrison GM,Sharov AA,Livigni A,Narayan S,Papapetrou EP,O'Malley J,Kaji K,Ko MS,Ptashne M,Brickman JM

更新日期：2012-02-23 00:00:00